RNA

Part:BBa_K199038:Design

Designed by: Romina Clemente   Group: iGEM09_MoWestern_Davidson   (2009-07-14)


pBad-CUAGU Suppressor tRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 176
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the pBad promoter to transcribe lower levels of tRNA in a high copy plasmid, pSB1A2.


Source

The pBad promoter is from the BioBrick Parts registry. Suppressor tRNA was made by de novo synthesis from single-stranded oligos. Based on the paper by Anderson et al. 2002.

References